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Calcium influx induces neurite growth through a Src-Ras signaling cassette

Domenii publicaţii > Biologie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Rusanescu G, Qi HQ, Thomas SM, Brugge JS, Halegoua S

Editorial: Neuron, 15 (6), p.1415-1425, 1995.

Rezumat:

We find that calcium influx through voltage-dependent calcium channels causes extensive neu rite outgrowth in PC12 cells. The calcium signal transduction pathway
promoting neurite outgrowth causes the rapid activation of protein tyrosine kinases, which include Src. Protein tyrosine phosphorylation results in the formation of an
Shc/Grb2 complex, reading to Ras activation, MAP kinase activation, and the subsequent induction of the immediate early gene NGFI-A. Protein tyrosine
phosphorylation, gene induction, and neurite outgrowth are inhibited by the expression of dominant negative forms of both Src and Ras, indicating a requirement for both
proto-oncoproteins in calcium signaling. Our results suggest that a signaling cassette which includes Src and Ras is likely to underlie it broad range of calcium actions in
the nervous system.

Cuvinte cheie: LONG-TERM POTENTIATION, PC12 CELLS, TYROSINE KINASES, NEURONAL DIFFERENTIATION, PHEOCHROMOCYTOMA CELLS,