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Electrochemical study of quercetin–DNA interactions: Part I.Analysis in incubated solutions

Domenii publicaţii > Chimie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Ana Maria Oliveira-Brett*, Victor C. Diculescu

Editorial: Bioelectrochemistry, 64, p.133, 2004.

Rezumat:

The present study aims to investigate the quercetin–deoxyribonucleic acid (DNA) interaction occurring in bulk solution either
electrochemically using differential pulse voltammetry or spectrophotometrically, in order to explain the possible DNA-damaging activity of
quercetin. A very weak interaction between quercetin and DNA in solution was found to take place. However, since extensive quercetininduced
DNA damage via reaction with Cu(II) has been reported, an electrochemical study of the DNA–Cu(II)–quercetin system in solution
was undertaken. The product of DNA interaction with quercetin–Cu(II) complex was observed. Damages to DNA were electrochemically
recognized via the increasing of the anodic peaks corresponding to the oxidation of guanosine and adenosine bases and
spectrophotometrically via increasing of the 260 nm adsorption band. It was also observed that dsDNA damage produced by the
quercetin–Cu(II) complex occurred with time. Control experiments with different mixtures of Cu(II), quercetin, ssDNA, dsDNA or poly[A]
were carried out in order to establish a possible mechanism of interaction between DNA and quercetin via Cu(II).

Cuvinte cheie: DNA-electrochemical biosensors; Quercetin; Oxidation; Electrochemistry; Radical scavenging activity; Flavonoids