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Formate Dehydrogenase-Modified Carbon Paste Electrodes for Amperometric Detection of Formate

Domenii publicaţii > Chimie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Florentina-Daniela Munteanu, Delia Gligor, Ionel Catalin Popescu and Lo Gorton

Editorial: Revue Roumaine de Chimie, 51(1), p.25-30, 2006.

Rezumat:

A simple and inexpensive amperometric biosensor for formate detection was obtained by immobilization of
formate dehydrogenase (FDH) on graphite powder, modified with a new phenothiazine derivative (DDDP; 16H,
18H-dibenzo[c,1]-7,9-dithia-16,18-diazapentacene). Two different enzyme environments were used: FDH was
adsorbed on DDDP-modified graphite powder in the presence of polyethylenimine (PEI) as well as in its absence. In
both cases, when 10 mM NAD+ were added in the supporting electrolyte (phosphate buffer, pH 7.0), the biosensor
developed a response to formate, obeying Michaelis-Menten kinetics with a linear domain up to 3 mM formate. The
biosensor sensitivity, calculated as the ratio Imax/KM
app, was better in the second case (0.962 mA/M), but the best
detection limit (3 µM) was observed in the first case.

Cuvinte cheie: formate dehydrogenase, carbon paste, biosensor