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Autori: Florentina-Daniela Munteanu, Francisco Ruiz-Dueñas, Angel Martínez, Artur Cavaco-Paulo
Editorial: Journal of Electroanalytical Chemistry, 580 (1), p. 35, 2005.
Electron transfer (ET) of versatile peroxidase (VP) was studied in the bioelectrocatalytic reduction reaction of H2O2 at peroxidase-modified graphite electrodes to obtain additional information on the kinetic characteristics of this novel ligninolytic peroxidase.
Rotating disk electrodes (RDE)experiments were performed at 0 V (vs. SCE) in two different buffers(tartrate buffer, pH 5.0; and citrate buffer, pH 3.0). From measurements of the mediated and mediatorless currents of H2O2 reduction at the RDE,the percentage of VP molecules involved in direct ET (½55%) was calculated. The peroxidase-modified electrodes were used for determination of the donor substrates in RDE mode, and the results were interpreted in terms of catalytic efficiencies.
Cuvinte cheie: Bioelectrocatalysis; Versatile peroxidase; Heterogeneous electron transfer; Graphite electrode