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In vitro cytotoxicity assessment of phthalocyanines on virus-transformed animal cells

Domenii publicaţii > Chimie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Radostina Alexandrova, Elena Stoykova, Rodica-Mariana Ion, Kristina Nedkova, Elena Ivanova, Kaloian Zdravkov, Georgi Minchev

Editorial: SPIE, Proc. SPIE Vol. 5830, p. 404-408,, 5830, p.404-408, 2005.


In this study we evaluate in vitro the cytotoxic activity of two newly synthesized phthalocyanines (metallo-complexes) with absorption bands in the red part of the spectrum: zinc-tri-sulphonated phthalocyanine (ZnS4Pc). Two permanent animal tumor cell lines are used in the experiments: LSR-SF(SR)-transplantable sarcoma in rat induced by Rous sarcoma virus strain Schmidt-Ruppin and LSCC-SF(Mc29)-transplantable chicken hepatoma induced by the myelocytomatosis virus Mc29. Irradiation has been performed with a laser diode emitting at 672 nm in a wide range of radiant exposure (2-100 J/cm2) and irradiance of 120 mW/cm2. The neutral red uptake cytotoxicity assay has been used to evaluate the effect of the photosensitizers on cell viability. The light dose-response curves and light exposures that ensure viability drop to 50% and 10% have been obtained for each cell line. The ability of the compounds to induce apoptosis has been determined by acridine orange dye staining

Cuvinte cheie: photodynamic therapy, porphyrins