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Autori: I. Bratu, M. T. Cotisel, G. Damian, A. Mocanu
Editorial: INOE&INFM, Journal of Optoelectronics and Advanced Materials, 9(3), p.675-677, 2007.
Structural information of barley aleurone at various temperatures is obtained by analysis of the conformationally-sensitive amide I band using FT-IR spectroscopy. The second derivative spectrum was performed in order to overcome the bands overlapping due to the different C=O stretching vibrations of each type of secondary structure (i.e. alpha-helix, beta-sheet, turns and unordered). The results of quantitative analysis by curve fitting to the inverted second derivative spectra indicate perturbations of both alpha-helix and beta-sheet structures in thermal unfolding process, depending on the temperature values.
Cuvinte cheie: FT IR spectroscopy, Secondary structure, Proteins, Barley aleurone, Deconvolution