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In vitro cytotoxicity assessment of second-generation photosensitizers for photodynamic therapy

Domenii publicaţii > Fizica + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: E. Stoykova, K. Nedkova, O. Sabotinov, R. Ion, R. Alexandrova

Editorial: J.Optoel.Adv.Mat., 9(2), p.490-493, 2007.


In this study we evaluate the cytotoxic activity of two phthalocyanines (metallo-complexes) with absorption bands in the red
part of the spectrum: zinc-tri-sulphonated phthalocyanine (ZnS3Pc) and zinc-tetrasulphonated phthalocyanine (ZnS4Pc) on
human (8 MG BA and MCF-7) and animal (LSCC-SF-Mc29 and LSR-SF-SR) tumor cell lines using a neutral red uptake
cytotoxicity test. The light source is a laser diode emitting at 672 nm. The experiments are made at fluences in the range (2
– 100 J/cm2) at fluence rate of 120 mW/cm2 chosen after a series of test measurements. The efficacy of both
phthalocyanines is evaluated from light dose response curves obtained for drug concentrations from 1 up to 10 μg/ml. Both
studied phthalocyanines exhibit effective treatment of the animal cell line LSCC-SF-Mc29 and the human cell line 8MG-BA.
In the case of ZnS3Pc, the cytotoxic effect is most distinguished for the tumor line 8MG-BA whereas for ZnS4Pc better
results are obtained for LSCC-SF-Mc29. The animal cell line LSR-SF-SR and the human line MCF-7 exhibit much greater
resistance to both photosensitizers at low concentration. Phthalocyanines (free-base or metallo-complexes), which are
synthetic porphyrin-like dyes are promising candidates among the second-generation PSs due to very large extinction
coefficients in the far red (660-700 nm).

Cuvinte cheie: Photodynamic therapy, Phthalocyanines, Survival light dose