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Analysis of marine microbial populations using molecular genetic tools: a new approach in the Romanian research at the Black Sea

Domenii publicaţii > Biologie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Elena Stoica

Editorial: Cercetari Marine - Recherches Marines, 36, p.203-212, 2006.

Rezumat:

Microorganisms represent the largest living surface in the marine environment and contain a vast diversity that are mainly undiscovered. Presently it is estimated that less than 1% of the marine microorganism can be cultured by classical cultivation techniques and most of the species have never been described. Several modern conceptual and methodological approaches based on the 16S rRNA culture-independent methods have been developed in the last decades and have provided enormous insights into the microbial diversity and activity of the most marine environments. Therefore, in the present paper the author discusses the necessity and perspectives of applying newly molecular genetic tools to study the complex microbial communities from the NW Black Sea coast, strongly affected by eutrophication over the last few decades. Particularly, the first data on the microbial taxonomic diversity obtained by using different molecular techniques (PCR amplification, sequencing of the 16S rRNA genes and fluorescence in situ hybridization) in both Romanian Black Sea water and sediments will be presented.

Cuvinte cheie: molecular genetic techniques, microbial populations diversity, Romanian coast, NW Black Sea