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An electrospray ionization mass spectrometric study of iron binding to amyloid-beta peptides

Domenii publicaţii > Chimie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Drochioiu, G.

Editorial: Eur. J. Mass Spectrom., 15, p.651-659, 2009.

Rezumat:

Iron and other metal ions appear to play an important role in protein aggregation and are therefore likely to provide a link between protein aggregation and oxidative damage. This work reports on iron binding to amyloid-beta peptide (Abeta1-40), which affords a very specific electrospray ionization mass spectrometric (MS) spectrum. Both MS and MS/MS study confirmed that amyloid beta-peptide displays a high affinity toward iron(III) ions, producing multi charged molecular ions and peptide aggregates. Finally, the circular dichroism spectra indicate an unexpected modification of Abeta1-40 peptide conformation upon iron binding.

Cuvinte cheie: iron-peptide complexes, electrospray ionization mass spectrometry, circular dichroism, iron chelation, amyloid-beta peptide

URL: http://www.ncbi.nlm.nih.gov/pubmed/19679945