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In vivo study regarding antioxidant effect of red grape polyphenol extract using biochemical and FT-IR methods

Domenii publicaţii > Fizica + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: L. I. SABAU, G. DAMIAN, D. DAICOVICIU, A. MURESAN, D. POSTESCU, D. MIHU, C. MIHU, N.COSTIN, C. ALB

Editorial: Journal of Optoelectronics and Advanced Materials-Symposia, 2(1), p.44-49, 2010.

Rezumat:

The aim of this study was to determine the effect of red grape polyphenol extract in menopause. The results showed that plasma level of reactive oxygen species (ROS) is significantly higher in ovariectomized rats, standardized food than in rats with false ovariectomy, standardized food. The plasma level of ROS decreased significantly in ovariectomized rats with supplemental grape polyphenol extract compared to ovariectomized rats, standardized food. The conclusion was that grape polyphenol extract reduces significantly the oxidative stress in ovariectomized rats. The investigation of the antioxidant effect of grape polyphenol extract, by studying the changes in secondary structure of plasma proteins in animals included in the study using attenuated total reflection Fourier transform infrared spectroscopy, was also completed. The statistically results reveal a decrease in α-helix and β-sheet content and an increasing of aggregates content for ovariectomized rats with standardized food compared with rats with false ovariectomy, standardized food. These changes are due to the oxidative stress that occurs in menopause. By administration of grape polyphenol extract in ovariectomized rats, it was observed an increased α-helix content as in rats with false ovariectomy, standardized food. Thus, the antioxidant effect of grape polyphenol extract was demonstrated as well using the FTIR spectroscopy.

Cuvinte cheie: Oxidative stress, Menopause, Grape polyphenols, Fourier transform and infrared spectroscopy.