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A Novel HPLC-PDA-MS Method for S-Adenosylmethionine and SAdenosylhomocysteine

Domenii publicaţii > Chimie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: C.Birsan, S.Litescu, G.L. Radu

Editorial: Taylor & Francis Group, Analytical Letters, 43(5), p.793-803, 2010.

Rezumat:

S-adenosylmethionine and S-adenosylhomocysteine are essential compounds in metabolic pathways; therefore, the development of a suitable method to routinely quantify these compounds is very important. In this paper, a simple and fast method (10 minutes) for simultaneous SAM and SAH quantification in plasma and blood samples is proposed based on the HPLC-PDA-MS technique. A new solid phase extraction procedure for blood samples is reported. The developed method proved to be highly sensitive(1.3109 molL1 for SAM and 4.51010 molL1 for SAH), repeatable (CV¼1.06% for SAM and CV¼0.92% for SAH), and reproducible (CV¼4.1% for SAM and CV¼3.77% for SAH).

Cuvinte cheie: HPLC; MS; S-adenosylhomocysteine; S-adenosylmethionine; SPE

URL: http://http://www.informaworld.com/smpp/title~content=t713597227