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Chronic venous insufficiency is associated with elevated level of circulating microparticles

Domenii publicaţii > Biologie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: A. Georgescu, N. Alexandru, D. Popov, M. Amuzescu, E . Andrei, C. Zamfir, H. Maniu, A. Badila

Editorial: Willey Blackwell, Journal of Thrombosis and Haemostasis, 7, p.1566-1575, 2009.


Background: Chronic venous insufficiency (CVI)results when the veins in the legs no longer pump blood back to the heart effectively. Microparticles (MPs) are small membrane
vesicles released by several circulating and vascular cells upon
activation or apoptosis. Objectives: The purpose of this study was to assess the subpopulations of circulating endothelial (EMPs) and platelet microparticles (PMPs) in CVI, and to
disclose their contribution in mediating dysfunction of human
peripheral venules. Patients and methods: Human peripheral venules were explanted during leg surgery on patients with CVI and on control subjects (C); concurrently, blood samples were collected and circulating MPs isolated. The techniques used were: flow cytometry, fluorescence and electron microscopy, myograph technique and western-blotting technique. Results: The results showed that compared with controls, patients with CVI had: (i) a marked elevation of
circulatingEMPsand PMPs; (ii) a structural modification of the venous wall consisting of activation of endothelial and smooth muscle cells, an abundance of intermediary filaments and
synthesis of hyperplasic-multilayered basal lamina; (iii) a significantly altered reactivity of the venous wall, closely associated with EMPs and PMPs adherence; (iv) altered contractile response to noradrenaline, acetylcholine, 5-hydroxytryptamine
and KCl, and an impeded relaxation in response to sodium nitroprusside; and (iv) a substantially increased protein
expression of tissue factor (TF) and of P-Selectin both in the venular vascular wall and on the surface of EMPs and
PMPs. Conclusions: The findings indicate that CVI is accompanied
by an enhanced release of EMPs and PMPs that contribute to altered dysfunctional response of the venous

Cuvinte cheie: disfunctia endoteliala, microparticule endoteliale,microparticule plachetare,reactivitatea vasculara a peretelui venos // endothelial dysfunction, endothelialmicroparticles, platelets microparticles, venous wall reactivity.