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Long-term high glucose concentration influences Akt, ERK1/2, and PTP1B protein expression in human aortic smooth muscle cells

Domenii publicaţii > Biologie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Doina Popov , Miruna Nemecz , Madalina Dumitrescu, Adriana Georgescu, Frank D. Böhmer

Editorial: Elsevier, Biochemical and Biophysical Research Communications , 388, p.51-55, 2009.

Rezumat:

Hyperglycemia stimulates a plethora of intracellular signaling pathways within the cells of the vascular
wall resulting in dysfunction-associated pathologies. Most of the studies reported so far explored the
effect of rather short-time exposure of smooth muscle cells to high glucose concentrations. To mimic situation
in Type 2 diabetes in which vascular wall is constantly exposed to circulating hyperglycemia, we
report here the long-term (7 days) effect of high glucose concentration on human media artery smooth
muscle cells. This consists in up-regulation of PTP1B protein expression, down-regulation of basal Akt
phosphorylation, and elevation of basal ERK1/2 activation. Acute stimulation of cells in high glucose with
insulin down-regulated PTP1B expression, slightly decreased ERK1/2 activity, and activated Akt, whereas
oxidative stress up-regulated Akt and ERK1/2 phosphorylation. In conclusion, long-term high glucose and
acute oxidative stress and insulin stimulation imbalance the expression of activated kinases Akt and
ERK1/2 and of dephosphorylating PTP1B in the insulin signaling pathway.

Cuvinte cheie: celule musculare netede din media aortei, hiperglicemie, calea de semnalizare a insulinei, kinaze, protein tirozin fosfataza 1B // Aortic media smooth muscle cells, Hyperglycemia, Insulin signaling, Kinases, Protein tyrosine phosphatase 1B

URL: www.elsevier.com/locate/ybbrc