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Probiotic colonisation of mucus-adherent HT29MTXE12 cells attenuates Campylobacter jejuni virulence properties.

Domenii publicaţii > Biologie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Abofu Alemka, Marguerite Clyne, Fergus Shanahan, Thomas Tompkins, Nicolae Corcionivoschi, and Billy Bourke

Editorial: Infection and Immunity, 2010.

Rezumat:

The HT29MTXE12 (E12) cell line harbours an adherent mucus layer, providing a novel technique to model mucosal infection in vitro. In this study, we have characterised the interaction of Campylobacter jejuni with E12 cells and exploited its unique mucus layer to examine the potential efficacy of probiotic treatment to attenuate C. jejuni virulence properties. C. jejuni 81-176 colonised and reproduced in E12 mucus. Adhesion to and internalisation of C. jejuni was enhanced in E12 cells harbouring mucus compared to parental cells without mucus. Translocation of C. jejuni occurred at early time points following infection. C. jejuni aligned with tight junctions and colocalised with the tight junction protein occludin, suggesting a paracellular route of translocation. Probiotic strains, Lactobacillus rhamnosus R0011, L. helveticus R0052, L. salivarius AH102, Bifidobacterium longum AH1205, a commercial combination of L. rhamnosus R0011 and L. helveticus R0052 (LacidofilTM), and a cocktail consisting of L. rhamnosus, L. helveticus and L. salivarius (RhHeSa), colonised E12 mucus and bound to underlying cells. Probiotics attenuated C. jejuni association with, internalisation into E12 cells and translocation to the basolateral medium of transwells. Live bacteria and prolonged precolonisation of E12 cells with probiotics were necessary for probiotic action. These results demonstrate the potential for E12 cells as a model of mucosal pathogenesis and provide a rationale for the further investigation of probiotics as prophylaxis against human campylobacteriosis.

Cuvinte cheie: campylobacter, colonisation, infection