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Production of a novel microbial transglutaminase using Streptomyces sp. polar strains

Domenii publicaţii > Biologie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Bahrim, G., Iancu, C., Buţu, N., Negoiţǎ, T.G.

Editorial: Romanian Biotechnological Letters , 15 (2), p.5197-5203, 2010.


Several biotechnological aspects regarding the increase in transglutaminase biosynthesis
yield were studied. The Streptomyces sp. strains used in this study, coded MIUG 6P, MIUG 7P and MIUG 13P, were isolated from soils from the East Antarctic coast. These had the best results in
terms of transglutaminase synthesis, thus confirming the results of a previous study concerning the
isolation and strains selection of good transglutaminase producers. Amongst these, strain coded MIUG 13P had the ability to produce 0.20 UA of transglutaminase per mL of crude extract, and was thus chosen to identify the optimal composition for the fermentative medium using Response surface methodology (RSM). Response surface methodology was further used to determine the optimum values of the process variables for enzyme production. The fit of the quadratic model was found to be significant. After submerged cultivation on the optimized fermentative medium, the enzymatic activity of the Streptomyces strain MIUG 13P showed a significant increase.

Cuvinte cheie: Microorganisme polare, enzyme, optimizare, metoda analizei suprafetei de raspuns // Polar microorganisms, enzymes, statistical optimization, response surface methodology