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Determination of ganglioside composition and structure inhuman brain hemangioma by chip-based nanoelectrospray ionization tandem mass spectrometry

Domenii publicaţii > Chimie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: C.Schiopu,C.Flangea,F.Capitan,A.Serb,Z.Vukelic, S.Kalanj-Bognar, E.Sisu, M. Przybylski, A.D.Zamfir

Editorial: springer, Anal Bioanal Chem, 395, p.2465-2477, 2009.

Rezumat:

We report here on a preliminary investigation of
ganglioside composition and structure in human hemangioma,
a benign tumor in the frontal cortex (HFC) in comparison to
normal frontal cortex (NFC) tissue using for the first time
advanced mass spectrometric methods based on fully
automated chip-nanoelectrospray (nanoESI) high-capacity
ion trap (HCT) and collision-induced dissociation (CID). The
high ionization efficiency, sensitivity and reproducibility
provided by the chip-nanoESI approach allowed for a reliable
MS-based ganglioside comparative assay. Unlike NFC,
ganglioside mixture extracted from HFC was found dominated
by species of short glycan chains exhibiting lower overall
sialic acid content. In HFC, only GT1 (d18:1/20:0), and GT3
(d18:1/25:1) polysialylated species were detected. Interestingly,
none of these trisialylated forms was detected in NFC,
suggesting that such components might selectively be
associated with HFC. Unlike the case of previously investigated
high malignancy gliosarcoma, in HFC one modified
O-Ac-GD2 and one modified O-Ac-GM4 gangliosides were
observed. This aspect suggests that these O-acetylated
structures could be associated with cerebral tumors having
reduced malignancy grade. Fragmentation analysis by CID
in MS2 mode using as precursors the ions corresponding to
GT1 (d18:1/20:0) and GD1 (d18:1/20:0) provided data
corroborating for the first time the presence of the common
GT1a and GT1b isomers and the incidence of unusual GT1c
and GT1d glycoforms in brain hemangioma tumor.

Cuvinte cheie: Gangliozide.hemangioma.Spectrometrie de masa in tandem.Biomarkeri tumorali // Gangliosides . Brain hemangioma . Chip-based nanoelectrospray . Tandem mass spectrometry . Brain tumor biomarker