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Kinetics aspects in the enzymatic hydrolysis of cellulose allomorphs

Domenii publicaţii > Chimie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Ciolacu, Diana; Ciolacu, Florin; Dumitriu, Raluca; Vasile, Cornelia; Popa, V. I.

Editorial: CELLULOSE CHEMISTRY AND TECHNOLOGY, 41 (1), p.37-42, 2007.


Although, within plant cells, the cellulose chains are organized in variable and complex ways, relatively
little attention has been paid to the relationship between this „fine-structure” of cellulose and its
biodegradation. This work focuses on the influence of the substrata and enzyme concentrations on the
enzymatic hydrolysis of microcrystalline cellulose. To determine the effect of the supramolecular structure
on the enzymatic degradation of cellulose, three crystalline forms of cellulose: I, II and III, have been
examined by systematic analysis of the behavior of these allomorphs during enzymatic hydrolysis; also, the
kinetics of biodegradation by the enzyme from Trichoderma reesei was investigated. The kinetic parameters
Km and Vmax were evaluated with the integral form of the Michaelis-Menten equation. The initial maximum
rate can be considered as a measure of substratum reactivity.

Cuvinte cheie: enzymatic degradation, Trichoderma reesei, cellulose allomorphs, kinetics