Autori: Thierry Noguer, Ana-Maria Balasoiu, Alina Avramescu & Jean-Louis Marty

Editorial: Analytical Letters, 34(4), p.513-528, 2001.

Rezumat:

A disposable monoenzymatic sensor for the reliable determination of dithiocarbamate fungicides was developed based on aldehyde dehydrogenase inhibition. Aldehyde dehydrogenase was immobilized on the surface of a disposable screen-printed carbon-paste electrode by using a photocross linkable poly-(vinylalcohol) bearing styrylpyridinium groups. Electrochemical oxidation of NADH was carried out at a potential as low as 0V vs Ag/AgCl using Meldola’s Blue as mediator. The sensor was used for the determination of metam-sodium, a soil fumigant, and its main metabolite MITC. It was shown that metam-sodium did not inhibit AlDH while MITC could be detected at levels of 100 ppb. The sensor thus allowed to discrimate between metam-sodium and its toxic metabolite MITC

Cuvinte cheie: analytical chemistry, electrochemistry, mass spectrometry, separations, spectroscopy,