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Possible role of apoptosis in the pathogenesis and clinical evolution of radicular cyst: an immunohistochemical study

Domenii publicaţii > Ştiinţe medicale + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Loreto C, Galanti C, Leonardi R, Musumeci G, Pannone G, Palazzo G, Rusu MC

Editorial: Int Endod J. , 46(7), p.642-8, 2013.

Rezumat:

AIM:

To investigate whether the apoptotic cascade is activated through the extrinsic pathway in epithelial lining and connective tissue of radicular cysts.
METHODOLOGY:

Fifteen radicular cysts were fixed in formalin, embedded in paraffin wax and processed for immunohistochemistry to evaluate the expression of polyclonal antibodies against Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL), DR5 and caspase-3. Immunocomplexes were treated with the secondary antibodies and finally detected using the avidin-biotin-peroxidase complex. Immunoreactivity was visualized by development with 3,3′-diaminobenzidine. Data were analysed using the Mann-Whitney U-test; P < 0.05 was considered significant. RESULTS: The three antibodies were detected in connective tissue fibroblasts of all radicular cysts; TRAIL and DR5 immunoexpression was significantly greater (P < 0.05) compared with that of caspase-3. The three antibodies were also expressed in almost all epithelial layers and in endothelial cells of newly formed vessels. CONCLUSION: The involvement of apoptosis in the pathogenesis of radicular cysts, demonstrated by the immunoexpression patterns of TRAIL, DR5 and caspase-3 in lining epithelium and connective tissue, may explain their bland clinical aggressiveness and slow, benign evolution.

Cuvinte cheie: apoptosis; TRAIL; caspase; DR5

URL: http://onlinelibrary.wiley.com/doi/10.1111/iej.12040/abstract;jsessionid=D7918C2B0275BF9290D06FE72DDA7013.f04t04