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Synchronous and periodic calcium oscillations in neuronal networks formed by sensory neurons in primary culture

Domenii publicaţii > Biologie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Radu BM, Radu M, Banciu DD

Editorial: Romanian Journal of Biophysics, 19(4), p.227-237, 2009.

Rezumat:

Neurite outgrowth was observed for the neuronal networks formed in primary neuronal cultures from dorsal root ganglia. Their activity was analyzed by fluorescence microscopy technique in order to evidence the level of calcium ions (calcium imaging) in cytosol of the neurons. Cells were loaded with calcium fluorescent dye Calcium Green-I. Using this technique; we have evidenced the spontaneous oscillations of the cytosolic calcium concentration in neurons coupled in a network. We have proved the synchronization and periodicity of these oscillations. The potassium chloride addition stimulates the neurons by producing a large and transient increase of the cytosolic calcium that proves the functionality of the neuronal network.

Cuvinte cheie: Neurite outgrowth, sensory neurons, calcium imaging, oscillations

URL: http://www.biophysicsnet.ro/rjb/articles/253/bmradu.pdf