Autori: Mihail Kantor, Radu Sestras and Kamal Chowdhury
Editorial: Pesquisa Agropecuária Brasileira (Brazilian Journal of Agricultural Research), volume 48, number 1, p.73-79, 2013.
The objective of this work was to obtain transgenic tomato plants expressing the PfCP2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day;old tomatoes,cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and
Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report
of successful transformation with the expression of a malaria antigen (PfCP2.9) in transgenic tomato plants
from the T0 and T1 generations.
Cuvinte cheie: modificare genetica, expresia genelor, malarie // gene expression, genetic transformation, malaria, plant‑made vaccines