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Artemisia plant’s constituents HPLC identification and their fungicide activity

Domenii publicaţii > Ştiinţe medicale + Tipuri publicaţii > Articol în volumul unei conferinţe

Autori: Diana Monica Obistioiu, Klaus Stolze, Ivo Schmerold, Cosmin Ioan Tuns, Ileana Nichita, Eugenia Dumitrescu, Diana Brezovan, Romeo Teodor Cristina

Editorial: Current Opinion in Biotechnology, 24, p.S114, 2013.


Candidiasis is a current topic, Candida albicans being structuresable to show a strong and extensive action, expressed with specific mycoses whose evolution are, often, train/rebellious to treatment. Aim of this study was to identify Artemisia plant constituents
with greater fungicidal effect than standard antifungal drugs used frequently in the treatment of candidiasis. Methodology included High Performance Liquid Chromatography (HPLC)
method, which was used to identify five standard compounds: linalool, cineole, cariophyllene, thujone and eugenole within different Artemisia essential oils. Also, the fungicidal effect of these standard compounds and their mixtures against clinical isolates of
C. albicans was followed using the Disc Diffusion Assay. Linalool was identified at 32.365 min; cineole is composed of an isomer
mixture and has produced a series of five peaks at: 41.485,
43.885, 44.780, and respectively 45.885 min. -Thujone generated a peak at 33.065 min, -cariophyllene, at 42.832 min and
eugenole at 27.740 min. Standards tested showed fungicidal effect as follows: eugenole > linalool > cariophyllene > thujone > cineole. Eugenole was by far the best antifungal standard tested, its
efficacy proving to be bigger by 32.2% to Nystatin and as a mixture; mixture eugenole + linalool + cariophyllene + thujone had an efficacy greater than Nystatin by +123.5%. After the in vitro tests done we can conclude that eugenol as single standard tested gave better results that the commonly used Nystatin but in mixtures its effect was +123.5% better that the allopathic drug.

Cuvinte cheie: Artemisia, HPLC, constituents, fungicide activity