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MAGNETIC NANOPARTICLES INFLUENCE ON SOME BACTERIAL CULTURES

Domenii publicaţii > Fizica + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: ANTONIEA POIATA, CRISTINA TUCHILUS, DORINA CREANGA, CRISTINA STAN

Editorial: Romanian Journal of Biophysics, 23(4), p.:203-209, 2013.

Rezumat:

Two suspensions of magnetic nanoparticles were prepared based on chemical coprecipitation of magnetite(MN1) and cobalt ferrite (MN2) from iron and respectively iron and cobalt salts, the magnetic powders being coated in sodium oleate for stabilization in deionized water. Microstructural and magnetic characterization was accomplished in both cases by applying standard methods. Two bacterial strains: Staphylococcus aureus – a Gram positive germ, and Escherichia coli – a Gram negative microorganism were cultivated in nutrient broth by adding 0.1 µL/mL magnetic nanoparticle suspension. Cell density was assessed by means of optical density measurements and calibration curve evidencing the inhibitory effect of magnetic nanoparticles on the bacterial cell growth. The diminution of S. aureus cell density was the most remarkable – more than 50% decrease compared to the control level for MN1 and MN2 test variants. E. coli response was consistent with smaller negative variations of the cell density following the treatment with magnetic nanoparticles – the cell density decreasing up to 93% for MN1 and MN2 samples.

Cuvinte cheie: metal oxides, human pathogens, cell density.

URL: http://www.biophysicsnet.ro/rjb/articles/382/art01Poiata.pdf

MAGNETIC NANOPARTICLES INFLUENCE ON SOME BACTERIAL CULTURES

Domenii publicaţii > Fizica + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: ANTONIEA POIATA, CRISTINA TUCHILUS, DORINA CREANGA, CRISTINA STAN

Editorial: Romanian Journal of Biophysics, 23(4), p.:203-209, 2013.

Rezumat:

Two suspensions of magnetic nanoparticles were prepared based on chemical coprecipitation of magnetite(MN1) and cobalt ferrite (MN2) from iron and respectively iron and cobalt salts, the magnetic powders being coated in sodium oleate for stabilization in deionized water. Microstructural and magnetic characterization was accomplished in both cases by applying standard methods. Two bacterial strains: Staphylococcus aureus – a Gram positive germ, and Escherichia coli – a Gram negative microorganism were cultivated in nutrient broth by adding 0.1 µL/mL magnetic nanoparticle suspension. Cell density was assessed by means of optical density measurements and calibration curve evidencing the inhibitory effect of magnetic nanoparticles on the bacterial cell growth. The diminution of S. aureus cell density was the most remarkable – more than 50% decrease compared to the control level for MN1 and MN2 test variants. E. coli response was consistent with smaller negative variations of the cell density following the treatment with magnetic nanoparticles – the cell density decreasing up to 93% for MN1 and MN2 samples.

Cuvinte cheie: metal oxides, human pathogens, cell density.

URL: http://www.biophysicsnet.ro/rjb/articles/382/art01Poiata.pdf

MAGNETIC NANOPARTICLES INFLUENCE ON SOME BACTERIAL CULTURES

Domenii publicaţii > Fizica + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: ANTONIEA POIATA, CRISTINA TUCHILUS, DORINA CREANGA, CRISTINA STAN

Editorial: Romanian Journal of Biophysics, 23(4), p.:203-209, 2013.

Rezumat:

Two suspensions of magnetic nanoparticles were prepared based on chemical coprecipitation of magnetite(MN1) and cobalt ferrite (MN2) from iron and respectively iron and cobalt salts, the magnetic powders being coated in sodium oleate for stabilization in deionized water. Microstructural and magnetic characterization was accomplished in both cases by applying standard methods. Two bacterial strains: Staphylococcus aureus – a Gram positive germ, and Escherichia coli
– a Gram negative microorganism were cultivated in nutrient broth by adding 0.1 µL/mL magnetic nanoparticle suspension. Cell density was assessed by means of optical density measurements and calibration curve evidencing the inhibitory effect of magnetic nanoparticles on the bacterial cell
growth. The diminution of S. aureus cell density was the most remarkable – more than 50% decrease compared to the control level for MN1 and MN2 test variants. E. coli response was consistent with smaller negative variations of the cell density following the treatment with magnetic nanoparticles – the cell density decreasing up to 93% for MN1 and MN2 samples.

Cuvinte cheie: metal oxides, human pathogens, cell density.

URL: http://www.biophysicsnet.ro/rjb/articles/382/art01Poiata.pdf