A disposable monoenzymatic sensor for the reliable determination of dithiocarbamate fungicides was developed based on aldehyde dehydrogenase inhibition. Aldehyde dehydrogenase was immobilized on the surface of a disposable screen-printed carbon-paste electrode by using a photocross linkable poly-(vinylalcohol) bearing styrylpyridinium groups. Electrochemical oxidation of NADH was carried out at a potential as low as 0V vs Ag/AgCl using Meldola's

An amperometric biosensor based on malate quinone oxidoreductase (MQO) was developed for monitoring of the malolactic fermentation of wines. Screen-printed electrodes coupled with appropriate mediators were used as transducers for this novel biosensor. MQO was immobilized by physical entrapment in a photo-cross-linkable poly(vinyl alcohol) polymer (PVA-SbQ) on the surface of the working electrode. Several electrochemical mediators were studied in

Amperometric biosensors based on NADHox/FMN and Prussian blue modified screen-printed electrodes (SPE) have been developed in order to improve the NADH detection. The catalytic effect, the sensitivity and the stability of PB-modified SPEs were studied for different pH buffer solutions and different voltage range, by cyclic voltammetry and amperometric measurements. NADH oxidase was immobilised on Prussian blue modified SPEs by entrapment in sol-gel,

Simple and low cost biosensor based on screen-printed electrode for sensitive detection of some alkylphenols was developed, by entrapment of HRP in a nanocomposite gel based on single-walled carbon nanotubes (SWCNTs) and 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM][PF6]) ionic liquid. Raman and FTIR spectroscopy, CV and EIS studies demonstrate the interaction between SWCNTs and ionic liquid. The nanocomposite gel, SWCNT–[BMIM][PF6] provides

Very sensitive, low cost and reliable NADH and H2O2 sensors were realised and used for development of enzyme based biosensors. The active surface of the electrodes was modified with a nanocomposite obtained by modification of SWNT with a proper mediator: Meldola Blue (for NADH) and Prussian Blue (for H2O2). Low applied potential of − 50 mV vs. Ag/AgCl reference electrode proved the synergistic effect of nanocomposite materials towards NADH and