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Oxidative damage following cerebral ischemia depends on reperfusion – a biochemical study in rat

Domenii publicaţii > Ştiinţe medicale + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Nita DA, Nita V, Spulber S, Moldovan M, Popa DP, Zagrean A-M, Zagrean L

Editorial: Journal of Cellular and Molecular Medecine, 5 (2), p.163-170, 2001.


The extent of brain injury during reperfusion appears to depend on the experimental pattern of ischemia/reperfusion.
The goals of this study were: first, to identify the rate of free radicals generation and the antioxidant activity during
ischemia and reperfusion by means of biochemical measurement of lipid peroxidation (LPO) and both enzymatic
(superoxid dismutase – SOD, catalase – CAT, glutathion peroxidase – GPx) and non-enzymatic antioxidants activity
(glutathione – GSH); and second, to try to find out how the pattern of reperfusion may influence the balance between
free radical production and clearance. Wistar male rats were subject of four-vessel occlusion model (Pulsinelly &
Brierley) cerebral blood flow being controlled by means of two atraumatic arterial microclamps placed on carotid
arteries. The level of free radicals and the antioxidant activity were measured in ischemic rat brain tissue homogenate
using spectrophotometrical techniques. All groups subjected to ischemia shown an increase of LPO and a reduction
of the activity of enzymatic antioxidative systems (CAT, GPx, SOD) and non-enzymatic systems (GSH). For both
groups subjected to ischemia and reperfusion, results shown an important increase of LPO but less significant than
the levels found in the group with ischemia only. Statistically relevant differences (p<0.01) between continuous reperfusion and fragmented reperfusion were observed concerning the LPO, CAT, SOD and GSH levels, oxidative aggression during fragmented reperfusion being more important.

Cuvinte cheie: free radicals, antioxidant enzymes, cerebral ischemia,