Cristina Purcarea

Institutul de Biologie , Bucuresti, .

E-mail: trimite un mesaj.

Nascut(a) in: 1965

Interese: biochimia proteinelor, extremofile, pirimidine, evolutie, adaptare, archaea

Detalii:
Biochimist specializat in biochimia proteinelor, cu expertiza in enzimologie, microbiologie, biologie moleculara si celulara, cristalografie de raze X.
SPECIALIZARE: (1) Studiul relatiei structura-functie a enzimelor provenind din microorganisme extremofile, in particular eubacterii si archaea hipertermofile si barofile, privind evolutia si mecanismele de adaptare la temperaturi si presiuni ridicate; (2) Microbiota intestinala si mecanisme moleculare implicate in obezitate

Details:
(1) Structure-function relationship of exzymes from extremophilic microorganisms; arginine and pyrimidine metabolisms from hyperthermophilic and barophilic archaea and eubacteria; evolution, adaptation mechanisms to high temperature and pressure. (2) Human gut microbiota, archaeal enzymes and their role in obesity

Publicații selectate:

* 8. Purcarea, C., Martin, P., Vickrey, J.F., Guy, H.I., Edwards, B.F.P., Evans, D.R. , Cloning, Expression and Preliminary X-ray Analysis of the Dihydroorotase from the Hyperthermophilic Eubacterium Aquifex aeolicus, Acta Crystallographica Section D Biological Crystallography, 58, 2002.

* Kothe, M., Purcarea, C., Guy, H., Evans, D.R., Powers-Lee, S, Direct Demonstration of Carbamoyl Phosphate Formation on the C-terminal Domain of Carbamoyl Phosphate Synthetase, Protein Sci, 14, 2005.

* Purcarea, C., Evans, D.R., Herve, G, Channeling of carbamoyl phosphate to the pyrimidine and arginine biosynthetic pathways in the deep-sea hyperthermophilic archaeon Pyrococcus abyssi, J Biol Chem, 274, 1999.

* Ahuja, A., Purcarea, C., Guy, H.I., Evans, D.R. , A novel carbamoyl phosphate synthetase from Aquifex aeolicus, J Biol Chem, 276, 2001.

* Purcarea, C., Lu, T., Ahuja, A., Kovari L., D.R. Evans, Aquifex aeolicus aspartate transcarbamoylase: an enzyme specialized for the efficient utilization of unstable carbamoyl phosphate at elevated temperature, J Biol Chem, 27, 2003.

* Zhang P, Martin PD, Purcarea C, Vaishnav A, Brunzelle JS, Fernando R, Guy-Evans HI, Evans DR, Edwards BF , Dihydroorotase from the hyperthermophile Aquifex aeolicus is activated by stoichiometric association with aspartate transcarbamoylase and forms a one-pot reactor for pyrimidine biosynthesis, Biochemistry, 48, 2009.

* Purcarea, C. Roshini, F., Guy, HI, Evans DR, The Sole Serine/threonine Protein Kinase and its Cognate Phosphatase from Aquifex aeolicus Targets Pyrimidine Biosynthesis, Mol Cell Biochem, 311, 2008.

* Cristina Purcarea, Aspartate transcarbamoylase from Pyrococcus abyssi, Acad. Press., M.W.W. Adams and R.M. Kelly, Methods in Enzymology, 331, 2001.