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Polyphenol oxidase immobilizatization on functionalized polypyrrole

Domenii publicaţii > Chimie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Teodor Sandu, Andrei Sârbu, Horia Iovu, Floriana Constantin, Emilia Ocnaru, Silviu Vulpe, Anca Dumitru

Editorial: Revue Roumaine de chimie, 56 (9), , p.875-882, 2011.

Rezumat:

Polypyrrole (PPY) was functionalized by reaction with glutardialdehyde using sulphuric acid as catalyst. The samples of functionalized polypyrrole were then used for the covalent immobilization of polyphenol oxidase. The surface elemental composition of this enzyme was determined by X-ray Photoelectron Spectroscopy. FTIR spectrometry, Raman spectroscopy, Scanning Electron Microscopy and X-Ray Diffraction were used to prove polypyrrole functionalization and the immobilization of this enzyme.

Cuvinte cheie: polipirol, imobilizare covalenta, polifenoloxidaza // polypyrrole, covalent immobilization, polyphenol oxidase