Tudorita (Doina) Tumbar

The focus of my laboratory is the understanding of basic mechanisms that regulate the fate of stem cells within tissues. We are currently studying skin and hair follicle stem cells using cell culture and the mouse as model systems. Hair follicle stem cells reside in their niche in a quiescent state, but become rapidly activated in response to homeostatic and injury signals to regenerate the tissue. We are analyzing the physiological role of several genes implicated in different aspects of stem cell activation, self-renewal, and differentiation. Our general focus is on stem cell fate choice by epigenetic regulation, transcription regulation via specific developmental transcription factors, and chromatin modifying factors. Additionally, we recently began to explore the potential implication of our genes of interest in cancer.

Cornell University, Ithaca, .

E-mail: trimite un mesaj.

Pagina web personala: http://tumbarlab.mbg.cornell.edu/Tumbar_lab_website/Home.html

Nascut(a) in: 1970

Interese: celule stem, genetica in soareci, transcriptie, cromatina

Detalii:
Laboratorul nostru studiaza mecanismele de baza implicate in deciziile celulelor stem de a se diferentia sau produce mai multe celule de acelasi fel. Cercetarea noastra foloseste pielea de soareci ca model de studiu. Celulele stem din par se gasesc intr-o zona specializata care functioneaza ca o nisa protectoare. Aceasta nisa prin semnalele ei biologice mentine celulele stem intr-o stare dormanta, temporar aflata in afara ciclului de diviziune. Aceste celule pot fi activate rapid sa se divida ca reactie la semnale biologice care promoveaza homeostazia tesutului si repararea ranilor. Grupul nostru analizeaza rolul catorva factori de transcriptie si factori epigenetici de remodelare a histonelor si structurii cromatinei. Unii dintre acesti factori sunt in aparenta implicati in cancerul de piele si cancerul oral.

Publicații selectate:

* Lee J, Hoi CS, Lilja KC, White BS, Lee SE, Shalloway D, Tumbar T., Runx1 and p21 synergistically limit the extent of hair follicle stem cell quiescence in vivo, PNAS, 110(12), 2013.

* Scheitz CJ, Lee TS, McDermitt DJ, Tumbar T., Defining a tissue stem cell-driven Runx1/Stat3 signalling axis in epithelial cancer, EMBO J, 31(21), 2012.

* Osorio KM, Lilja KC, Tumbar T., Runx1 modulates adult hair follicle stem cell emergence and maintenance from distinct embryonic skin compartments., J Cell Biol, 193(1), 2011.

* Zhang YV, White BS, Shalloway DI, Tumbar T., Stem cell dynamics in mouse hair follicles: a story from cell division counting and single cell lineage tracing., Cell Cycle, 9(8), 2010.

* Hoi CS, Lee SE, Lu SY, McDermitt DJ, Osorio KM, Piskun CM, Peters RM, Paus R, Tumbar T., Runx1 directly promotes proliferation of hair follicle stem cells and epithelial tumor formation in mouse skin., Mol Cell Biol, 30(10), 2010.

* Zhang YV, Cheong J, Ciapurin N, McDermitt DJ, Tumbar T., Distinct self-renewal and differentiation phases in the niche of infrequently dividing hair follicle stem cells, Cell Stem Cell, 5(3), 2009.

* Waghmare SK, Bansal R, Lee J, Zhang YV, McDermitt DJ, Tumbar T., Quantitative proliferation dynamics and random chromosome segregation of hair follicle stem cells, EMBO, 27(9), 2008.

* Tumbar T, Belmont AS., Interphase movements of a DNA chromosome region modulated by VP16 transcriptional activator., Nat Cell Bio, 2(12), 2001.

* Tumbar T, Sudlow G, Belmont AS., Large-scale chromatin unfolding and remodeling induced by VP16 acidic activation domain., J Cell Biol, 145(7), 1999.

* Osorio KM, Lee SE, McDermitt DJ, Waghmare SK, Zhang YV, Woo HN, Tumbar T., Runx1 modulates developmental, but not injury-driven, hair follicle stem cell activation., Development, 6, 2008.

* Tumbar T, Guasch G, Greco V, Blanpain C, Lowry WE, Rendl M, Fuchs E., Defining the epithelial stem cell niche in skin., Science, 303 (5656), 2004.