Laura-Dorina Dinu

Universitatea Agronomica - Facultatea de Biotehnologii, Bucuresti, .

E-mail: trimite un mesaj.

Pagina web a instituţiei: http://bioteh.usab.ro

Nascut(a) in: 1970

Interese: microbiologie, genetica microbiana, raspunsul microbian la stres

Detalii:
2004 - 2005 Bursa Marie Curie, Universitatea din Nottingham (UK). Lucrarea de dizertatie "Rezistenta heterogena la stresul salin in drojdii" s-a referit la raspunsul biologic la stresul salin in microorganismul model eucariot Saccharomyces cerevisiae folosind tehnici de inginerie genetica si citometrie in flux.
2005 Teza de doctorat "Modele microbiene si toleranta la stresul salin in plante" a explorat mecanismul molecular care asigura heterogenitatea genetica si fenotipica in raspunsul drojdiilor la stresul salin, pentru a genera un model care poate fi extins la plantele superioare (drojdiile si plantele prezinta un mecanism similar de adaptare la stresul salin)
2006 - 2009 Bursa postdoctorat, PARC-Summerland (Canada). "Riscul de contaminare a produselor horticole cu celulele induse de stres VBNC ale bacteriei E. coli O157:H7" a investigat generarea, transmiterea si virulenta celulelor tip VBNC ale patogenilor umani. S-au utilizat tehnici de inginerie genetica (clonare, secventiere,QPCR), microbiologice si de microscopie electronica (CSLM).

Details:
2004 - 2005 Marie Curie Fellow, University of Nottingham (UK). Dissertation work "Heterogeneous resistance to salt stress in yeast" focused on the biological response to salt stress in the eukaryotic model Saccharomyces cerevisiae using genetic engineering and flow cytometry techniques.
2005 PhD thesis "Microbial models and salt stress tolerance in plants" explored the molecular mechanism for genetic and phenotypic heterogeneity in salt stress response to yeast in order to design a model that may be extended to higher plants (both share the same adaptation mechanism to salt stress).
2006 - 2009 NSERC Postdoctoral VF Fellow, PARC-Summerland (Canada)."The risk posed by stress-induced viable but non-culturable (VBNC) E. coli O157:H7 in contamination of fresh vegetables" has investigated the food safety concern associated with the VBNC cells of human pathogens using molecular (gene cloning, DNA sequencing, QPCR), culture and microscopy (CSLM) techniques.

Publicații selectate:

* L-D. Dinu, P. Delaquis, S. Bach, Nonculturable response of animal enteropathogens in the agricultural environment and implications for food safety, Journal of Food Protection, vol. 72, no. 6, 2009.

* P. Delaquis, S. Bach, L-D. Dinu, Behavior of Escherichia coli O157:H7 in leafy vegetables, Journal of Food Protection, vol. 70, no. 8, 2007.