Autori: A. Georgescu, N. Alexandru, M. Nemecz, I. Titorencu, D. Popov

Editorial: Blood Coagulation and Fibrinolysis, 22(4), p.310-316, 2011.

Rezumat:

The arterial endothelial dysfunction in aging and diabetes remains a clinical problem. We questioned the effect of the low-molecular-weight heparin, enoxaparin, on arterioles contractility in aging and in aging associated with diabetes, and investigated the involvement of the mitogen-activated protein (MAP) kinases pathway in the enoxaparin-mediated effect.
The experiments were performed on the isolated resistance arteries of young (4 months old), aged (16 months old), and aged-diabetic hamsters (16 months old and 5 months since streptozotocin injection). The techniques used were myography, molecular biology, and immunoblotting.
The results showed that 60 micrograms per ml enoxaparin has favorable effects on the arteriole reactivity in aged and aged-diabetic conditions, reducing the contractile response to 10-10 mol/l noradrenaline. The diminishment of contractility is exerted via MAP kinase pathway, and involves reduction of c-fos gene expression and of transcription factor AP-1 protein expression. These results suggest that enoxaparin preserves the arterial endothelial function in a mechanism independent of its anticoagulant activity.
Understanding the signal transduction mechanisms involved in the altered contractility of vascular wall could provide useful information on the development of specific MAP kinase inhibitors with therapeutic benefits and reduced side effects.

Cuvinte cheie: aging, arterial wall contraction, diabetes, enoxaparin, MAP kinase

Autori: Adriana Georgescu, Nicoleta Alexandru, Miruna Nemecz, Irina Titorencu and Doina Popov

Editorial: Blood Coagulation and Fibrinolysis, 22(4), p.310:316 , 2011.

Rezumat:

The arterial endothelial dysfunction in aging and diabetes remains a clinical problem. We questioned on the effect of
the low-molecular-weight heparin, enoxaparin, on arterioles contractility in aging and in aging associated with diabetes, and investigated the involvement of mitogen-activated protein (MAP) kinases pathway in the enoxaparin-mediated effect. The experiments were performed on the isolated resistance arteries of young (4 months old), aged(16 months old), and aged-diabetic hamsters (16 months
old and 5 months since streptozotocin injection). The techniques used were myography, molecular biology, and immunoblotting. The results showed that 60mg/ml enoxaparin has favorable effects on the arterioles reactivity in aged and aged-diabetic conditions, reducing the contractile response to 10S8-10S6 mol/l noradrenaline. The diminishment of contractility is exerted via MAP kinase
pathway, and involves reduction of c-fos gene expression and of transcription factor AP-1 protein expression. These results suggest that enoxaparin preserves the arterial endothelial function in a mechanism independent of itsanticoagulant activity. Understanding the signal transduction mechanisms involved in the altered contractility of vascular wall could provide useful information on the development of specific MAP kinase inhibitors with therapeutic benefits and reduced side effects.

Cuvinte cheie: arterioles, aiging, diabetes