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TRANSGENIC PLUMS EXPRESSING PLUM POX VIRUS COAT PROTEIN GENE DO NOT ASSIST THE DEVELOPMENT OF VIRUS RECOMBINANTS UNDER FIELD CONDITIONS

Domenii publicaţii > Biologie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: I. Zagrai, M. Ravelonandro, I. Gaboreanu, B. Ferencz, R. Scorza, L. Zagrai, B. Kelemen, D. Pamfil, O. Popescu

Editorial: Journal of Plant Pathology, 93(1), p.159-165, 2011.

Rezumat:

The serological and genetic variability of Plum pox
virus (PPV) isolates from transgenic plum trees expressing
the PPV coat protein gene and conventional plums
was analyzed. PPV isolates were characterized serologically
by TAS-ELISA using PPV-D and PPV-M specific
monoclonal antibodies and by molecular typing across
three genomic regions as well as RFLP analysis of the 3’
terminus of the CP gene. PCR products spanning the
(Cter)CP and (Cter)NIb-(Nter)CP regions were sequenced.
Sequence information revealed no significant
difference between serological and molecular features of
PPV isolates from transgenic and conventional plums
that were growing together in experimental orchards for
6-8 years. These results show that transgenic plums do
not promote the emergence of new PPV variants under
field conditions.

Cuvinte cheie: pathogen derived resistance, GMO, CP gene, safety issue, recombinant viruses