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EPR study of noncovalent spin labelled serum albumin and haemoglobin

Domenii publicaţii > Fizica + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: S.Cavalu, G.Damian, M.Dânşoreanu

Editorial: Elsevier, Biophysical Chemistry, 99:2, p.181-188, 2002.


Electron Paramagnetic Resonance (EPR) was used to investigate the Tempyo spin label (3-carbamoyl-2,2,5,5-tetramethyl-3-pyrrolin-1-yloxy) as a report group for the interactions and the conformational changes of lyophilized bovine serum albumin (BSA) and bovine haemoglobin (BH), as function of pH. values in the range 2.5÷11. The EPR spectra are similar with those of other noncovalently spin label porphyrins in frozen solution at very low temperatures. This behavior indicated a possible spin-spin interaction between the hemic iron and the nitroxide group. The changes in the EPR spectra as function of the pH are discussed in terms of conformational changes of the proteins. Spectral simulations and magnetic EPR parameters reveal the following: (i) one single paramagnetic species, with Gaussian line shape, was used for the best fits of experimental spectra in the case of serum albumin samples, and (ii) a weighted sum of Lorentzian and Gaussian line shape in the case of hemoglobin samples. The representation of correlation time versus pH, reveals a dependence of degree of immobilization of spin label on the conformational changes of proteins in acidic and bazic environment.

Cuvinte cheie: EPR, spin label, serum albumin, haemoglobin