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Vesicle traffic through intercellular bridges in DU 145 human prostate cancer cells

Domenii publicaţii > Ştiinţe medicale + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: C. Vidulescu, S. Clejan, K.C.O'Connor

Editorial: Journal of Cellular and Molecular Medicine, 8, p.388-396, 2004.


We detected cell-to-cell communication via intercellular bridges in DU 145 human prostate cancer cells by fluorescence microscopy. Since DU 145 cells have deficient gap junctions, intercellular bridges may have a prominent role in the transfer of chemical signals between these cells. In culture, DU 145 cells are contiguous over several cell diameters through filopodial extensions, and directly communicate with adjacent cells across intercellular bridges. These structures range from 100 nm to 5 micrometers in diameter, and from a few micrometers to at least 50 – 100 micrometers in length. Time-lapse imagery revealed that (1) filopodia rapidly move at a rate of micrometers per minute to contact neighboring cells and (2) intercellular bridges are conduits for transport of membrane vesicles (1-3 micrometers in diameter) between adjacent cells. Immunofluorescence detected alpha-tubulin in intercellular bridges and filopodia, indicative of microtubule bundles, greater than a micrometer in diameter. The functional meaning, interrelationship of these membrane extensions are discussed, along with the significance of these findings for other culture systems such as stem cells. Potential applications of this work include the development of anti-cancer therapies that target intercellular communication and controlling formation of cancer spheroids for drug testing.

Cuvinte cheie: intercellular bridges, nanotubes, filopodia, membrane vesicles, intercellular communication, prostate cancer