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Structural differences of porphyrins in photodynamic therapy induce ditinct antineoplastic effects

Domenii publicaţii > Chimie + Tipuri publicaţii > Articol în volumul unei conferinţe

Autori: M.Neagu, G.Manda, C.Constantin, RM Ion

Editorial: Elsevier, Journal of Porphyrins and Phthalocyanines, 10, p.788, 2006.


The aim of the study was to investigate the influence of discrete structural differences in synthetic porphyrins on the antineoplastic effect induced in lymphoblastic cell line K562 after experimental photodynamic therapy procedure (PDT). For this study, it was synthesized 5,10,15,20-tetra-1-naphthyl-porphyrin (TNP), 5,10,15,20-tetra-p-sulphonatophenyl-porphyrin (TSPP) and their Zn complexes (ZnTSPP or ZnTNP). For all these compounds, it was optimized the following parameters: cell concentration, dye loading concentration, time of loading and irradiation procedure. The non-toxic doses of porphyrins were different according to their structure: for TNP compounds, the dose was of 10microg/mL, and for TSPP compounds, the dose was 20microg/mL. The cell functionality was assesed as membrane integrity, viability, number of metabollicaly active cells. The photodynamic kinetics of cell suspensions showed that during irradiation, tumor cells are actively destroyed comparatively with unloaded tumor cells. K562 cell line loaded with the above mentioned compounds and subjected to irradiation display lower proliferative capacity compared to control, the reduced proliferative capacity is maintained 72h after irradiation. The proliferative capacity of K562 cell line is more strongly inhibited by the TNP family of compounds in comparison with the TSPP family. The tested synthetic porphyrins (TNP and TSPP families) have efficient antineoplastic activity in the in vitro photodynamic therapy experimental model.

Cuvinte cheie: experimental photodynamic therapy, K562 cell line, porphyrins, zinc porphyrins