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Synthetic porphyrins in experimental photodynamic therapy induce a different anti tumoral effect

Domenii publicaţii > Chimie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: M. Neagu, G. Manda, C. Constantin, E. Radu, R.M.Ion

Editorial: Elsevier, J. Porphyrins Phthalocyanines, 11(1), p.58-65, 2007.


The aim of the study was to investigate the influence of discrete structural differences
in synthetic porphyrins on the antineoplastic effect induced in myelocitic cell line K562 after experimental photodynamic therapy procedure. For this study, we used 5,10,15,20-tetra (1-na phthyl)-
porphyrin, 5,10,15,20-tetra (4-sulfonatophenyl)porphyrin and their Zn complexes. For all these compounds, the following photodynamic therapy parameters were optimized: cell concentration,dye loading, concentration, loading time and irradiation procedure. The non-toxic doses of porphyrins were different according to their structure: for 5,10,15,20-tetra (1-naphthyl) porphyrin compounds,the dose was of 10 microg.mL-1, and for 5,10,15,20-tetra (4-sulfonatophenyl) porphyrin compounds, the dose was 20 microg.mL-1. Cell functionality was assessed as membrane integrity, viability, and number of metabolically active cells. Photodynamic cell degradation kinetics showed that during irradiation
tumor cells are actively destroyed, in contrast to unloaded cells. K562 cells loaded with the abovementioned
compounds and subjected to irradiation, displayed lower proliferative capacity com pared
to the control, for a period of 72 h after irradiation. The proliferative capacity of K562 cell line was
more strongly inhibited by the 5,10,15,20-tetra(1-naphthyl) porphyrin family of compounds than by
the 5,10,15,20-tetra (4-sulfonatophenyl) porphyrin family. The tested synthetic porphyrins showed
effective antineoplastic activity against K562 leukemia cells in our in vitro photo dynamic therapy
experimental model.

Cuvinte cheie: photodynamic therapy, K562 cell line, porphyrins, zinc complexes.