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Rat osseous plate alkaline phosphatase as Langmuir monolayer—An infrared study at the air–water interface

Domenii publicaţii > Chimie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: L. Caseli, D. C. Masui, R. P.M. Furriel, F. A. Leone, M. E.D. Zaniquelli, J. Orbulescu and R. M. Leblanc

Editorial: Elsevier, Journal of Colloid and Interface Science, 302 (2), p.476-482, 2008.

Rezumat:

A glycosylphosphatidylinositol (GPI)-anchored enzyme (rat osseous plate alkaline phosphatase—OAP) was studied as monolayer (pure and mixed with lipids) at the air–water interface. Surface pressure and surface potential–area isotherms showed that the enzyme forms a stable monolayer and exhibits a liquid-expanded state even at surface pressure as high as 30 mN/m. Isotherms for mixed dimyristoylphosphatidic acid (DMPA)–OAP monolayer showed the absence of a liquid-expanded/liquid-condensed phase transition as observed for pure DMPA monolayer. In both cases, pure or mixed monolayer, the enzyme preserves its native conformation under compression at the air–water interface as observed from in situ p-polarized light Fourier transform-infrared reflection–absorption spectroscopic (FT-IRRAS) measurements. Changes in orientation and conformation of the enzyme due to the presence or absence of DMPA, as well as due to the surface compression, are discussed.

Cuvinte cheie: rat osseous plate alkaline phosphatase, Langmuir monolayer, surface chemistry, IRRAS

URL: http://dx.doi.org/10.1016/j.jcis.2008.01.043