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Multi-modal imaging of cytosolic and mitochondrial Ca2+

Domenii publicaţii > Stiinte ingineresti + Tipuri publicaţii > Articol în volumul unei conferinţe

Autori: Ciubotaru CD, Bastianello S, Beltramello M, Pozzan T, Mammano F

Editorial: M.H. Hamza, ACTA Press, Anaheim, CA, USA, Proceedings of the IASTED International Conference on Biomedical Engineering, p.99-102, 2005.


Cultured HeLa cells were loaded with Ca sensitive
fluorescent probes targeted to the mitochondria (rhod-2), and
to the cytosol (fluo-3 and fura-2). We performed
fluorescence imaging on these live cells under software
control and enhanced the optical sectioning off-line by
applying a nearest-neighbor deconvolution algorithm based
on a directly estimated point-spread function (PSF). The
different dynamics of cytosolic and mitochondrial Ca2+
signals evoked the UV flash photolysis of a photo-labile Ca2+
chelator could be distinguished clearly, with sub-micron
resolution. The results indicate that this approach is suitable
for the analysis of the interactions and cross-talks between
different signaling pathways.

Cuvinte cheie: Optical imaging, deconvolution, bioinformatics, biomedical computing, calcium dynamics