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Alpha-methylacyl-CoA racemase, a novel specific biomarker for prostate cancer

Domenii publicaţii > Ştiinţe medicale + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: R. O. Dumache, B. G. Bumbacila, A. Anghel, F. Miclea, M. Puiu

Editorial: nature publishing group, European journal of human genetics, Volume 16 Supplement 2 May 2008, p.232, 2008.


Alpha-methylacyl-CoA racemase, abbreviated in data bases as AMACR or P504S, is a 382 amino acid protein that plays a critical role in peroxisomal and mitochondrial beta oxidation of branched chain fatty acid and bile acid intermediates, dihydroxycholestanoic acid and trihydroxycholestanoic acid, molecules. Specifically, it catalyses the conversion of (R) a – methyl branched chain fatty acyl CoAs to their (S) stereoisomers, because only stereoisomers with the 2-methyl group in the (S) position can be metabolized by peroxisomal oxidases, the first enzymes in the ß oxidation pathway. It is well known that the main sources of branched chain fatty acids are the dairy products and red meet pork and beef, the consumption of which has been associated with an increased risk for prostate cancer in men, in multiple studies.
We evaluated the AMACR gene expression in 32 patients aged 55 to 80, with total PSA values in a range of 2 to 40 ng/ml. 26 patients were diagnosed positive for prostate intraepithelial carcinoma and they had PSA values greater than 11 ng/ml. In these 26 men, we found by using PCR and micro array techniques on needle biopsies that they had an over expressed gene for AMACR, too. The other 6 patients with total PSA values smaller than 11 ng/ml, had normal expression of the AMACR gene.
According to our results, we propose the use of AMACR as an important marker of prostate cancer in Urology Clinics beside PSA and morphopathological diagnostic.

Cuvinte cheie: Alpha-methylacyl-CoA racemase, marker, cancer de prostata // Alpha-methylacyl-CoA racemase*marker*prostate cancer