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Comparison of various sensitive and selective spectrophotometric assays of environmental cyanide

Domenii publicaţii > Chimie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Drochioiu, G., Popa, K., Humelnicu, D., Murariu, M., Sandu, I., Cecal, A

Editorial: Toxicol. Environ. Chem., 90(2), p.221-235, 2008.


A review is presented to show the advantages involved in the use of some new spectrophotometric cyanide (CN)assays, which are highly sensitive and selective, quick and affordable. Among these, the ninhydrin-based assay, the method based on CN reaction with resorcinol, as well as the Berthelot reaction were found to be highly selective. The old Aldridge 1 method, based on the formation of cyanogen bromide and its subsequent reaction with pyridine and benzidine to form a highly-colored polymethine chromophore is still in use, although recent assays using isonicotinic acid-sodium barbiturate are safe, selective, and sensitive (with a detection limit of 0.005-0.01 mg L-1 CN). Ninhydrin-based procedures are suitable to detect low trace amounts of CN with a molar absorptivity below 2.2 times 105 L M-1 cm-1 and detection limit of less than 1 ng cm-1 CN. The sensitivity, specificity, and reliability of the investigated methods were compared and the most appropriate protocol for a given analytical problem described.

Cuvinte cheie: Cyanide determination, spectrophotometry, ninhydrin, Nagashima method, environment monitoring