Autori: Drochioiu, G., Manea, M., Dragusanu, M., Murariu, M., Dragan, E. S., Petre, B. A., Mezo, G., Przybylski, M.

Editorial: Biophys. Chem., 144, p.9-20, 2009.

Rezumat:

The stoichiometries and the affinity toward simple and paired metal ions of synthetic amyloid-beta(1-40)
peptide (Abeta1-40) were investigated by electrospray ion trap mass spectrometry (ESI-MS), circular dichroism CD), and atomic force microscopy (AFM). The results lead to the working hypothesis that pH-dependent
metal binding to Abeta 1-40 may induce conformational changes, which affect the affinity toward other metals.
A significant copper and zinc binding to Abeta 1-40 peptide at pH 5.5 was found, whereas nickel ions commonly
bind to each molecule of beta-amyloid peptide. Some complexes of Abeta1-40 with more than one nickel ion were
identified by ESI-MS. In addition, nickel ions proved to enhance Abeta oligomerization. On increasing pH, up to
12 ions of zinc may bind to a single Abeta molecule. Under the same pH and concentration conditions, the
binding pattern of the independent copper and silver ions to Abeta 1-40 was different from that of the
equimolecular mixture of the two metal ions. One might assume that some conformational changes due to
water loss altered the capacity of Abeta peptide to bind certain heavy metal ions. As a consequence, copper-
silver interaction with the binding process to Abeta 1-40 became highly complex. A competition between silver
and nickel ions for Abeta 1-40 binding sites at high pH was also observed. New strategies were proposed to
identify the characteristic signals for some important metal ion-peptide complexes in the spectra recorded at
high pH or high concentrations of metal ions. To explain the formation of such a large number of high metal
ion-Abeta complexes, we took into consideration the participation of both histidine residues and free amino
groups as well as carboxylate ones in the binding process. Finally, CD and AFM studies supported the mass
spectrometric data.

Cuvinte cheie: Amyloid beta;-peptide, Metal ion-peptide complex, Alzheimer's disease, ESI ion trap MS

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