Autori: A.Zamfir, N.Dinca, E.Sisu, J.Peter-Katalinic

Editorial: WILEY-VCH, J. Sep. Sci., 29(3), p.414-422, 2006.

Rezumat:

A sturdy home-built sheathless CE/ESI-QTOF-MS system was developed and optimized
for carbohydrate analysis. The interface and employed methodology provided a
simple analytical solution to laborious CE/MS interfacing methods and to problems
in characterization of complex carbohydrate mixtures that require high-resolution
separation of the components. The CE/ESI interface, feasible in any MS laboratory,
consists of a one-piece CE column having the CE terminus in-laboratory shaped as a
microsprayer and coated with copper. The CE microsprayer was inserted into a inhouse
made stainless steel clenching device and the whole assembly was mounted
onto a quadrupole TOF mass spectrometer. The analytical potential of the interface,
in terms of suitability, microsprayer performance, copper coat durability, ionization
efficiency, spray stability, and sensitivity, was tested first on a simple mixture
of standard saccharides, which were separated, resolved, and detected with high
separation efficiency. The approach was next assessed for the screening of a biological
sample, a complex mixture of O-glycosylated sialylated amino acids from urine
of a patient suffering from Schindler disease. Preliminary data allow to consider
this method as one of general applicability in structural glycobiology and glycomics
and easy to be implemented for proteomic surveys as well.

Cuvinte cheie: Glicopeptide/monozaharide/spectrometrie de masa /QTOF/Ionizare prin electrospray // Glycopeptides / Monosaccharides / Quadrupole time-of-flight mass spectrometry / Sheathless capillary electrophoresis / Electrospray ionization