Biglycan (BGN) is a small proteoglycan that consists of a protein core containing leucine-rich repeat regions and two glycosaminoglycan (GAG) chains of either chondroitin sulfate (CS) or dermatan sulfate (DS) type. The development of novel, highly efficient analytical methods for structural identification of BGN-derived CS/DS motifs, possibly implicated in biological events, is currently the focus of research. In this work, an improved analytical

Gangliosides (GGs), sialic acid-containing glycosphingolipids, are known to be involved in the invasive/metastatic behavior of brain tumor cells. Development of modern methods for determination of the variations in GG expression and structure during neoplastic cell transformation is a priority in the field of biomedical analysis. In this context, we report here on the first optimization and application of chip-based nanoelectrospray (NanoMate robot)

An original strategy was set-up in order to develop new azo compounds which incorporate in their structure 4,4'- diaminodiphenylmethane (DADFM) and low molecular weight maltodextrine. The synthesis of the model compounds involved the functionalisation of DADFM with the maltodextrine, the conversion of amino functionalized maltodextrine in a diazonium salt and the coupling of reaction with H and R acids. The compounds were characterized by UV-VIS,

The present work focused on the design of a drug delivery system (DDS) based on pH-sensitive hydrogel. The hydrogels were prepared via graft copolymerization of mixtures of acrylic acid (AA) and 2-hydroxy ethyl methacrylate (HEMA) onto starch backbones by a free radical polymerization technique. Sodium bicarbonate (NaHCO3) was added to function as a foaming agent under acidic conditions, rendering the hydrogels to be porous. The porous structure

In the past few years, a considerable effort was invested in interfacing mass spectrometry (MS) to microfluidics-based systems for electrospray ionization (ESI). Since its first introduction in biological mass spectrometry, chip-based ESI demonstrated a high potential to discover novel structures of biomarker value. Therefore, recently, microfluidics for electrospray in conjunction with advanced MS instruments able to perform multistage fragmentation

The biochemical role of metals in urolithogenesis is explained by their participation in the processes of coprecipitation, as a consequence of perturbations in metabolism and urodynamics. Under the conditions of a metastable medium, represented by renal excretion, alkaline and alkaline-earth metals (with predilection) participate in the mechanism of heterogeneous nucleation were organic and inorganic metabolites eliminated by kidney. Our investigations

Le decelement des metaux transitionnels dans la composition des calculs justifie l'interet croissant pour l'etude de leur participation aux processus d'urolithogenese. La coprecipitation se produit durant la nucleation heterogene ( organique et inorganique) dans le milieu metastable represente par l'excretion renale. Les investigations ont ete effectues sur 119 calculs, extraits par interventions chirurgicales ou elimines spontanement.Apres avoir

This paper presents the synthesis of an asymmetric dysazoic direct dye containing 4,4'-Diaminobenzanilide as middle component and salicylic acid respectively 2-benzoylamino-5-hidroxy-7-naphtalene sulfonic acid as coupling components. The dye was characterized by thin layer chromatography (TLC), electronic spectra (VIS) and Mass spectroscopy (FABS). Coloristic and fastness properties were determined too. Using this dye, a comparative study of its

Dimethyl acetals of mono and dinitrobenzophenones are easily prepared in quantitative yield by treatement with methanol and dimethylsulfite in the presence of continous hidrogen chloride stream. The new acetals were fully characterized by 1H-NMR, 13C-NMR,IR spectroscopy, MS(EI) and elemental analysis.