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Thin layer chromatography separation of some carotenoids, retinoids and tocopherols

Domenii publicaţii > Chimie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Rodica Domnica Briciu, Dorina Casoni, Cristina Bischin

Editorial: Studia Universitatis Babes-Bolyai, Chemia, 4, p.67-76, 2008.


A simple and reliable thin layer chromatography (TLC) method
for simultaneous separation of some carotenoids (β-carotene, lycopene,
lutein, astaxanthin and zeaxanthin), retinoids (retinol, retinoic acid, 9-cisretinal
and all-trans-retinal) and tocopherols (α-tocopherol, γ-tocopherol
and δ-tocopherol) was described. For a proper separation of all compounds
three different mobile phases (Benzene: Petroleum Ether, 10:90, v/v;
Methanol: Benzene: Ethyl acetate, 5:75:20, v/v/v; Benzene: Petroleum
Ether, 90:10, v/v) were tested, using silica gel F254 as stationary phase. The visualization was performed under UV light (254 and 365 nm). The presented
method is simple and adequate for the separation and identification of
these compounds from different matrices, such as pharmaceuticals and foods.

Cuvinte cheie: Carotenoide, Retinoide, Tocoferoli, Cromatografie pe strat subtire // Carotenoids, Retinoids, Tocopherols, Thin layer chromatography