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HPTLC Quantification of Some Flavonoids in Extracts of Satureja hortensis L. Obtained by Use of Different Techniques

Domenii publicaţii > Chimie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Ildiko Bros, Maria-Loredana Soran, Rodica Domnica Briciu, Simona Codruta Cobzac

Editorial: Journal of Planar Chromatography - Modern TLC, 22 (1), p.25-28, 2009.


A study of quantitative analysis of some flavonoids in Satureja hortensis
L. extracts is presented. The HPTLC analyses were performed
in a normal chromatographic chamber on silica gel plates, with an
appropriate mobile phase. Photodensitometric evaluation of the
chromatographic plates was performed by UV–visible absorbance
at 320 nm for rosmarinic acid and 349 nm for luteoline, after pulverizing
with NTS reagent (diphenylboryloxyethylamine). Quantitative
analyses of flavonoids were performed by use of calibration
plots. The quantity of compounds extracted depends on the composition
of the extraction solvent and on the extraction technique. The
best results were obtained by heating under reflux with systems that
contained 60–70% ethanol. Under these conditions it was found that
this plant contained approximately 34 mg g–1 rosmarinic acid and
30 mg g–1 luteoline. The results obtained were compared and correlated
with extraction method and confirm this plant contains important
amounts of flavonoids and phenolic acids.

Cuvinte cheie: HPTLC, Flavonoide, Satureja hortensis L, Tehnici de extractie // HPTLC, Flavonoids, Satureja hortensis L, Extraction techniques