Inscriere cercetatori

EPR investigation of libration motion of spin labeled hemerythrin

Domenii publicaţii > Fizica + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Istvan Mihaly Takacs, Augustin Mot, Radu Silaghi-Dumitrescu, Grigore Damian

Editorial: Elsevier, Journal of Molecular Structure,, 2014.


Reported here are room-temperature continuous wave X-band Electron Paramagnetic Resonance (EPR) spectra of the non-heme di-iron protein hemerythrin (Hr), spin labeled at position 51C in different viscous media, illustrating the mobility and oligomeric recombination tendency of the Phascolopsis gouldii Hr. The mobility of a spin labeled Hr depends on the local viscosity and its connectivity to the nature of the molecular environment (glycerol, PEG4000 and BSA). This provides the basis for a tool useful in directly monitoring Hr in ex vivo samples upon injection within the bloodstream of test animals, for blood substitute research.

Cuvinte cheie: Spin labeling; EPR spectroscopy; Hemerythrin