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Behavior of mixed DMPC-cholesterol monolayers at the air/water interface

Domenii publicaţii > Chimie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Mocanu, A. , Paşca, R.-D., Horovitz, O., Tomoaia-Cotisel, M.

Editorial: Studia Universitatis Babes-Bolyai Chemia, 2 (2), p.303-312, 2010.


The phase behaviour of cholesterol (CHO) and dimyristoyl phosphatidyl choline (DMPC) monolayers at the air/water interface was investigated by surface pressure measurements using Langmuir technique. Compression isotherms and isobars as functions of composition were represented, and the surface compressibility modulus was calculated, in order to characterize the physical states of the monolayers. At a constant lateral surface pressure, the monolayers were transferred by Langmuir-Blodgett technique (LBT) from air-water interface on solid supports and further studied by atomic force microscopy (AFM). This study shows that the strong interactions between DMPC and CHO lead to well defined twodimensional nanostructures, which can have practical applications for biosensors fabrication. Also, this type of nanostructures seems plausible to occur in natural membranes and thus, can influence the protein distribution and protein function in vivo.

Cuvinte cheie: Atomic force microscopy; Cholesterol; DMPC; Langmuir-blodgett technique; Monolayers