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Ferencz, A; Juhasz, R; BUTNARIU, M; Deer, AK; Varga, IS; Nemcsok, J. Expression analysis of heat shock genes in the skin, spleen and blood of common carp (Cyprinus carpio) after cadmium exposure and hypothermia, Acta Biologica Hungarica, Volume: 63, Issue: 1, Pages: 15–25, Published: MAR 2012 (Accession Number: WOS: 000302010900002, PubMed ID: 22453797, ISSN: 0236–5383), Times Cited: 13 (from Web of Science Core Collection), IF (2014): 0.589.
ACTA BIOLOGICA HUNGARICA, ISSN: 0236-5383, Volume: 63, Issue: 1, Pages: 15-25, DOI: 10.1556/ABiol.63.2012.1.2, Published: MAR 2012
Abstract: Heat shock proteins are chaperones that play a pivotal role in controling multiple regulatory pathways such as stress defense, hormone signaling, cell cycle control, cell proliferation and differentiation, and apoptosis. In this study, the expression patterns of four well-known heat shock genes (hsp70, hsc70-1, hsc70-2 and hsp90 alpha) were characterized in the skin, spleen and blood cells of the common carp, under unstressed conditions and after Cd2+ treatment or hypothermia. The examined genes were expressed in a tissue-specific manner: hsc70-2 was expressed constitutively, and was at best only slightly inducible; hsp90 alpha exhibited a high basic expression in all three tissues, whereas hsc70-1 did so only in the blood cells, the expression of hsp70 proved to be below the level of detection in unstressed fish. Cold shock induced the expression of hsp genes in the spleen (hsp90 alpha) and blood cells (hsp70, hsc70-1 and hsp90 alpha), while Cd2+ treatment has no effect on the expression pattern. The highest inducibilities were detected in the skin: for hsp70 an induction of at least 20-fold after cadmium exposure, for hsc70-1 of at least 30-fold and for hsp90 alpha of 3-fold after hypothermia.
Web of Science Categories: Biology