Autori: Constantin Munteanu, Diana Ciobotaru, Gabriela Zamfirescu, Otilia Zarnescu, Lotus Mester

Editorial: EDS B. GRUEV, M. NIKOLOVA AND A. DONEV, PROCEEDINGS OF THE BALKAN SCIENTIFIC CONFERENCE OF BIOLOGY, p.P. 612-624, 2005.

Rezumat:

Glial cells are no longer considered to be passive structural and trophic supports for neurons, but rather play important and integral roles in the physiological functioning of the central nervous system in both health and disease. Lithium effects on in vitro models have been the subjects of many studies since lithium was recognized as an effective drug in the treatment of bipolar disorder. The primary glial cells culture was initiated from the brain of Wistar rats aged 3-4 days. In Romania, there are some sources of mineral waters, which contain between 3-8 mg Li+/l. One of these waters is Mary Source from Malnaş-Bãi, with 8.03 mg Li+/l. The purpose of this study is to observe how is changed the morphological aspect of cultured glial cells after treatment with lithium chloride or lithium mineral waters. After 12 days, morphological changes caused by lithium cytotoxicity occur in culture such as cell swelling, cytoplasm granulation, and vacuolisation. Using lithium mineral waters to a dilution of ¼, we obtained a positive effect for the development of glial cells culture. When we added 1mM LiCl and lithium mineral waters to a dilution of ¼, the effect was still positive for the culture. The efficacy of lithium mineral waters was tested by compeering control glial cells, cultivated in standard medium, with those cultivated in medium with lithium mineral waters or in the presence of lithium chloride in various concentrations.

Cuvinte cheie: lithium, glial cells, lithium mineral waters, bipolar disorder

URL: http://web.uni-plovdiv.bg/mollov/bio/bscb2005/part1/612-624.pdf