Tempyo labelled bovine serum albumin and cytochrome c at different pH values were prepared and investigated using Raman/resonance Raman and SERS spectroscopy. Raman spectra of tempyo labelled proteins in the pH range from 6.7 to 11 were compared to those of the corresponding free species. SERS spectra were interpreted in terms of adsorption-induced structural changes of tempyo labelled proteins on the silver colloidal surface. Tempyo spin label was

Betulinic acid [BA, 3b-hydroxy-20(19)-lupaen-28-oic acid] can be chemically derived from betulin, a substance found in abundance in the outer bark of white birch trees (Betula alba). This molecule is a pentacyclic triterpene, difficult to be solved for spectroscopic experiments. The bark which this birch sheds naturally and causes its white appearance, abundantly provides the BA in the form of betulin. BA has been found to induce apoptosis, cell