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Electrophysiological and neurochemical techniques to investigate sensory neurons in analgesia research

Domenii publicaţii > Biologie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Babes A, Fischer MJ, Reid G, Sauer SK, Zimmermann K, Reeh PW

Editorial: Springer, Methods in Molecular Biology, 617, p.237-259, 2010.


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The primary afferent nociceptive neuron has recently attracted major research interest because of the cloning of very selectively expressed and well-conserved ion channel genes. All parts of the neuron, sensory terminals, axon and cell body, are accessible to validated research techniques in vitro using various isolated tissues or cells taken from laboratory animals. Single-unit recording and measuring stimulated calcitonin gene-related peptide (CGRP) release as well as patch-clamping and calcium imaging of cultured sensory neurons provide different kinds of information, and no model alone answers all questions. In combination, however, consistent results and complementary evidence form a solid basis for translational research to follow.

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Cuvinte cheie: patch clamp, skin nerve, pain, inflammation